Figure 2From: Genetic visualization of the secondary olfactory pathway in Tbx21 transgenic miceOrganization of mouse Tbx21 gene and transgene constructs. (A) Genomic organization of Tbx21 gene on mouse chromosome 11. Exons are indicated by boxes with numbers: purple boxes indicate the open reading frame; and light blue boxes represent 5'- and 3'-non-translated regions. (B) Nucleotide sequence identity plots of human, rhesus, dog and rat Tbx21 genes compared with mouse ortholog by using the VISTA program [41]. Highly conserved regions (> 80% identity) in 5'-flanking sequences and introns are shown in pink. Mitral and tufted cell-specific enhancer (MCE; 307 bp) is present at ~3 kb upstream from the transcription start site of Tbx21 gene. (C) Transgene constructs. 5'-flanking regions of Tbx21 gene with different lengths (5.0, 2.6 and 1.0 kb: yellow) were fused to a membrane-targeted Venus complementary DNA (cDNA) (gV: green) to generate Tbx5.0gV, Tbx2.6gV and Tbx1.0gV transgenes. Highly conserved 307-bp sequence (MCE: red) and SV40 minimal promoter (blue) were fused with gapVenus cDNA to generate MCE-gV transgene. 5.0-kb 5'-flanking region of Tbx21 gene was fused with synaptopHluorin cDNA (spH: dark blue) in order to generate Tbx-spH transgene.Back to article page