Figure 4

Diverse neuronal architectures of the ventral local interneurons (vILNs). Single cell vlLN clones showing examples of (A-D) ipsilaterally and (E-F) bilaterally projecting neurons. (A) An ipsilaterally innervating oligoglomerular neuron (arborizing in one to three glomeruli). (B-D) Ipsilaterally innervating multiglomerular (arborizing in a large subset of glomeruli) neurons. (A-D) Arbours terminate in contiguous glomeruli (continuous innervation). (E) Bilaterally innervating LN. The innervation is multiglomerular on the ipsilateral side with only oligoglomerular (one to three) innervation contralaterally. (F) Bilaterally innervating neuron with oligoglomerular arbours in both antennal lobes. (A1, B1, C1, D1, E1 F1) Neuron labelled with the mCD8::GFP reporter. (C1, D1, E1, F1) Tiled images made from cropped portions of appropriate sections to highlight the single cell. (A2, B2, C2, D2, E2, F2) Merged images (of GFP and Brp channels) that are reconstructed (A3, B3, C3, D3, E3 and F3) of confocal sections through the antennal lobe using Amira. White arrowheads indicate the cell bodies. Images are oriented as shown in (B3): D = dorsal, L = lateral. Scale bars = 10 μm.