The ventrolateral glutamatergic cell cluster is born from a single lineage. (A) MARCM-derived neuroblast clone labelled with Gal4-OK371 generated by heat shock at 12 ALH. (A1, A2) A cluster of cells (yellow arrowheads) located ventrolateral to the antennal lobe (demarcated by white dots) is labelled. (A2) The antennal glomeruli were labelled with staining with mAbnc82 (Brp) (magenta). (A1 inset) A few confocal sections stacked to enable clearer visualization of somata. (A1, A2) Commissural contralateral projections (indicated by white arrow). (B) Confocal sections were used to reconstruct the interneuron cluster using Amira http://www.amira.com. Scale bar = 10 μm. Images are oriented as shown in (A2): D = dorsal; L = lateral.